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Amylase production by preussia minima, a fungus of endophytic origin: optimization of fermentation conditions and analysis of fungal secretome by LC-MS

Source: BMC Microbiology 2014

Environmental screening programs are used to find new enzymes that may be utilized in large-scale industrial processes. Among microbial sources of new enzymes, the rationale for screening fungal endophytes as a potential source of such enzymes relates to the hypothesised mutualistic relationship between the endophyte and its host plant.

There is a need for new microbial amylases that are active at low temperature and alkaline conditions as these would find industrial applications as detergents.

Results: An alpha-amylase produced by Preussia minima, isolated from the Australian native plant, Eremophilia longifolia, was purified to homogeneity through fractional acetone precipitation and Sephadex G-200 gel filtration, followed by DEAE-Sepharose ion exchange chromatography. The purified alpha-amylase showed a molecular mass of 70 kDa which was confirmed byzymography.

Temperature and pH optima were 25[degree sign]C and pH 9, respectively. The enzyme was activated and stabilized mainly by the metal ions manganese and calcium.

Enzyme activity was also studied using different carbon and nitrogen sources. It was observed that enzyme activity was highest (138 U/mg) with starch as the carbon source and L-asparagine as the nitrogen source.

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